POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING

Mihai CENARIU; Ioan GROZA; Liviu Bogdan; Emoke PALL; Simona  CIUPE

doi:10.52331/cvj.v15i1.1

Authors

Mihai CENARIU University of Agricultural Sciences and Veterinary Medicine
Ioan GROZA University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Veterinary Reproduction, Obstetrics and Gynecology
Liviu Bogdan University of Agricultural Sciences and Veterinary Medicine
Emoke PALL University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Veterinary Reproduction, Obstetrics and Gynecology
Simona CIUPE University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Veterinary Reproduction, Obstetrics and Gynecology

DOI:

https://doi.org/10.52331/cvj.v15i1.1

Keywords:

bovine embryo, biopsy, polymerase chain reaction, fluorescent in situ hybridization

Abstract

The purpose of this paper was to evaluate the results obtained for bovine embryo sexing using the polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) and to compare them in order to decide which of the two methods is more accurate and yields better results while necessitating less effort. We took into consideration the pregnancy rate obtained after the transfer of biopsied embryos, the percentage of correctly sexed embryos evaluated at birth (when the predicted sex was compared with the actual sex of the newborn) as well as other characteristics related to the difficulty of the method, expenses and suitability to a minimally equipped laboratory. We concluded that the polymerase chain reaction is the most accurate and suitable method for sexing preimplantation bovine embryos, being in the same time easier to perform than the fluorescence in situ hybridization.

References

Bredbacka P. – Progress on methods of gene detection in preimplantation embryos. Theriogenology 55 pp 23-34, 2001.

Cenariu M., I. Groza, R. Al. Pop, Brînduşa Stegeran, Emoke Pall, Laura Cătană, A. Bartoş - Bovine embryo sexing using the fluorescence in situ hybridization (FISH), Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Veterinary Medicine, 65 (2)/2008, ISSN 1843-5270, pp 109-113, 2008.

Cenariu M., I. Groza, Simona Ciupe, Brânduşa Stegeran, Emoke Pall, Cătană Laura, A. Bartoş - Bovine embryo sexing using the polymerase chain reaction (PCR), Scientific Papers USAMV Iaşi, Veterinary Medicine, vol. 51/2008, ISSN 1454-7406, pp 252-256, 2008

Groza I., L. Bogdan, I. Morar, Simona Ciupe, R. Pop, M. Cenariu, C. Peştean - Cercetari privind variantele de inovulare a embrionilor la bovine, Clujul Medical Veterinar, nr.8, pp 8-12, 2005.

GrozaI.şi col.–Ginecologie,AndrologieşiObstetricăVeterinară,Ed.AcademieiRomâne,Bucureşti,2006

Lee J.H., J. H. Park S.H. Lee, C.S. Park, D. I. Jin - Sexing using single blastomere derived from IVF bovine embryos by fluorescence in situ hybridization (FISH), Theriogenology 62, pp 1452-1458, 2004

JinL., R.V. Lloyd-Insituhybridization: methodsandapplications. J.Clin.Lab.Anal.11(1),pp2-9,1997

Shea B.E. - Determining the sex of bovine embryos using polymerase chain reaction results: a six-year retrospective study. Theriogenology 51, pp785-797, 1999.

Taketo, T., Lee, C.H., Zhang, J., Li, Y., Lee, C.Y., Lau, Y.F. - Expression of SRY proteins in both normal and sex-reversed XY fetal mouse gonads. Dev. Dyn. 233, pp 612–622, 2005.