Comparative evaluation of florfenicol and polymeric nanopar-ticles loaded with florfenicol against bacterial strains isolated from chickens: insights into antimicrobial resistance

Authors

  • Emilia Trif
  • Constatin Cerbu University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Infectious Diseases
  • Marina spinu
  • Diana Ioana Olah
  • Adrian Valentin Potarniche
  • Sergiu-Dan Zablau
  • Florina Marian
  • George Hertanu
  • Emoke Pall
  • Gheorghe Florinel Brudasca

DOI:

https://doi.org/10.52331/cvj.v28i1.43

Keywords:

florfenicol, antibiotic-loaded nanoparticles, antimicrobial resistance

Abstract

Antimicrobial resistance (AMR) poses a significant threat to both human and animal health, necessitating the search for alternative antimicrobial agents and strategies. In this study, we aimed to identify and isolate clinical bacterial strains from chickens and evaluate their sensitivity to florfenicol, a common antimicrobial agent that is used exclusively in veterinary medicine, along with polymeric nanoparticles loaded with florfenicol at various concentrations. Three clinical bacterial strains were successfully isolated and identified from chicken presenting clinical signs. In order to assess their susceptibility, the isolated strains were subjected to a standard disc diffusion assay using florfenicol. Subsequently, polymeric nanoparticles loaded with florfenicol were tested at six different concentrations and compared their efficacy against the bacterial strains. Our results demonstrated that all three clinical bacterial strains exhibited varying degrees of resistance to florfenicol. Interestingly, the use of polymeric nanoparticles loaded with florfenicol did not display enhanced antimicrobial activity compared to the free drug. Notably, the efficacy of the loaded nanoparticles did not significantly vary with different concentrations of active substance. This study highlights the importance of exploring novel therapeutic approaches to combat antimicrobial resistance. The use of polymeric nanoparticles loaded with florfenicol presents a promising avenue for overcoming resistance mechanisms and improving the efficacy of antimicrobial treatments both in human and veterinary medicine. Further investigations are needed to elucidate the underlying mechanisms and optimize the formulation of polymer nanoparticles for enhanced therapeutic outcomes in combating AMR.

References

L. Garcia-Migura, R. S. Hendriksen, L. Fraile, and F. M. Aarestrup, “Antimicrobial resistance of zoonotic and commensal bacteria in Europe: The missing link between consumption and resistance in veterinary medicine,” Veterinary Microbiology, vol. 170, no. 1–2. Elsevier, pp. 1–9, 2014. doi: 10.1016/j.vetmic.2014.01.013.

J. L. Watts, M. T. Sweeney, and B. V. Lubbers, “Current and future perspectives on the categorization of antimicrobials used in veterinary medicine,” J Vet Pharmacol Ther, vol. 44, no. 2, pp. 207–214, Mar. 2021, doi: 10.1111/jvp.12846.

R. R. Watkins and R. A. Bonomo, “Overview: The Ongoing Threat of Antimicrobial Resistance,” Infectious Disease Clinics of North America, vol. 34, no. 4. W.B. Saunders, pp. 649–658, Dec. 01, 2020. doi: 10.1016/j.idc.2020.04.002.

E. Palma, B. Tilocca, and P. Roncada, “Antimicrobial resistance in veterinary medicine: An overview,” International Journal of Molecular Sciences, vol. 21, no. 6. MDPI AG, Mar. 02, 2020. doi: 10.3390/ijms21061914.

L. Cantas et al., “A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota,” Front Microbiol, vol. 4, no. MAY, 2013, doi: 10.3389/fmicb.2013.00096.

A. Mateus, D. Brodbelt, and K. Stärk, “Evidence-based use of antimicrobials in veterinary practice,” In Pract, vol. 33, no. 5, pp. 194–202, May 2011, doi: 10.1136/inp.d2873.

L. Tollefson and W. T. Flynn, “Impact of Antimicrobial Resistance on Regulatory Policies in Veterinary Medicine: Status Report,” 2002. [Online]. Available: http://www.aapspharmsci.org

P. L. Toutain, A. A. Ferran, A. Bousquet-Melou, L. Pelligand, and P. Lees, “Veterinary medicine needs new green antimicrobial drugs,” Frontiers in Microbiology, vol. 7, no. AUG. Frontiers Research Foundation, Aug. 03, 2016. doi: 10.3389/fmicb.2016.01196.

J. Espinasse, “Responsible use of antimicrobials in veterinary medicine: perspectives in France,” 1993.

N. R. Naylor et al., “Estimating the burden of antimicrobial resistance: a systematic literature review,” Antimicrob Resist Infect Control, vol. 7, p. 58, 2018, doi: 10.1186/s13756-018-0336-y.

M. Ismail and Y. A. El-Kattan, “Comparative pharmacokinetics of florfenicol in the chicken, pigeon and quail,” Br Poult Sci, vol. 50, no. 1, pp. 144–149, Jan. 2009, doi: 10.1080/00071660802613286.

O. Hassanin, F. Abdallah, and A. Awad, “Effects of florfenicol on the immune responses and the interferon-inducible genes in broiler chickens under the impact of E. coli infection,” Vet Res Commun, vol. 38, no. 1, pp. 51–58, Mar. 2014, doi: 10.1007/s11259-013-9585-7.

S. AL-Shahrani and V. Naidoo, “Florfenicol induces early embryonic death in eggs collected from treated hens,” BMC Vet Res, vol. 11, no. 1, Aug. 2015, doi: 10.1186/s12917-015-0536-0.

X. Li, G. Wang, X. Du, B. Cui, S. Zhang, and J. Shen, “Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among isolates from diseased chickens,” vol. 8, pp. 243–247, 2007.

A. Bello, B. Poźniak, A. Smutkiewicz, and M. Świtała, “The influence of the site of drug administration on florfenicol pharmacokinetics in turkeys,” Poult Sci, vol. 101, no. 1, Jan. 2022, doi: 10.1016/j.psj.2021.101536.

X. Mei et al., “Florfenicol Enhances Colonization of a Salmonella enterica Serovar Enteritidis floR Mutant with Major Alterations to the Intestinal Microbiota and Metabolome in Neonatal Chickens PUBLIC AND ENVIRONMENTAL HEALTH MICROBIOLOGY,” 2021. [Online]. Available: https://www.cdc.gov/

E. A. H. Abu-Basha, R. Gehring, A. F. Al-Shunnaq, and S. M. Gharaibeh, “Pharmacokinetics and bioequivalence of florfenicol oral solution formulations (Flonicol® and Veterin®10%) in broiler chickens,” J Bioequivalence Bioavailab, vol. 4, no. 1, pp. 0001–0005, 2012, doi: 10.4172/jbb.1000101.

A. Brauner, O. Fridman, O. Gefen, and N. Q. Balaban, “Distinguishing between resistance, tolerance and persistence to antibiotic treatment,” Nat Rev Microbiol, vol. 14, no. 5, pp. 320–330, 2016, doi: 10.1038/nrmicro.2016.34.

A. Anadón et al., “Plasma and tissue depletion of florfenicol and florfenicol-amine in chickens,” J Agric Food Chem, vol. 56, no. 22, pp. 11049–11056, Nov. 2008, doi: 10.1021/jf802138y.

Downloads

Published

2023-06-17

How to Cite

“Comparative evaluation of florfenicol and polymeric nanopar-ticles loaded with florfenicol against bacterial strains isolated from chickens: insights into antimicrobial resistance” (2023) Cluj Veterinary Journal, 28(1), pp. 14–19. doi:10.52331/cvj.v28i1.43.

Similar Articles

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)