The Histopathological and macroscopical considerations of induced experimental periodontitis in rats

Authors

  • Stefana Muresan Usamv Cluj
  • Alexandra Dreanca University of Agricultural Sciences and Veterinary Medicine
  • Andras Nagy University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur no 3-5, 400372, Cluj-Napoca, România
  • Calin Repciuc University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur no 3-5, 400372, Cluj-Napoca, România
  • Robert Cristian Purdoiu University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur no 3-5, 400372, Cluj-Napoca, România
  • Alexandru Raul Pop University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur no 3-5, 400372, Cluj-Napoca, România
  • Stelian Pantea University of Oradea, Universității street, no 1, 410087 Oradea, România
  • Liviu Oana University of Agricultural Sciences and Veterinary Medicine

DOI:

https://doi.org/10.52331/cvj.v27i2.41

Keywords:

chronic inflamation, bone necrosis, alveolar bone loss, rats, experimental periodontitis

Abstract

Ten Wistar males rats were used to induce experimental periodontitis by placing a 5-0 cotton thread ligature at the base of the first superior molar on the left side. Before this phase, the molar went through the process of scaling, rooting and planning. Soft movements of the molar were realized for creating an accumulation of plaque by flattening and by resulting the displacement of the gingival tissue, thus provoking an inflammatory response. After 7 days the ligatures were removed in all ten rats. After 14 days, results obtained showed gross aspects of periodontitis and microscopical lesions as well, installed in the periodontium. Microscopically, an inflammatory response with bone necrosis and alveolar bone loss was observed. The aim of this study is to test an experimental protocol of periodontitis confirming the presence of this pathology by gross aspects and also histopathological aspects. In conclusion, the tested procedure is able to provide all the key biological elements present in periodontal disease, whereas representing the adequate features for biomaterial testing domain.

References

Lorencini, M.; Silva, J.A.F.; Almeida, C.A.; Bruni-Cardoso, A.; Carvalho, H.F.; Stach-Machado, D.R. A New Paradigm in the Periodontal Disease Progression: Gingival Connective Tissue Remodeling with Simultaneous Collagen Degradation and Fibers Thickening. Tissue Cell 2009, 41, 43–50, doi:10.1016/J.TICE.2008.07.001.

Goyal, G.; Garg, T.; Rath, G.; Goyal, A.K. Current Nanotechnological Strategies for an Effective Delivery of Drugs in Treatment of Periodontal Disease. Critical Reviews™ in Therapeutic Drug Carrier Systems 2014, 31, 89–119, doi:10.1615/CRITREVTHERDRUGCARRIERSYST.2014008117.

Prates, R.A.; Yamada, A.M.; Suzuki, L.C.; França, C.M.; Cai, S.; Mayer, M.P.A.; Ribeiro, A.C.; Ribeiro, M.S. Histomorphometric and Microbiological Assessment of Photodynamic Therapy as an Adjuvant Treatment for Periodontitis: A Short-Term Evaluation of Inflammatory Periodontal Conditions and Bacterial Reduction in a Rat Model. Photomed Laser Surg 2011, 29, 835, doi:10.1089/PHO.2010.2984.

Struillou, X.; Boutigny, H.; Soueidan, A.; Layrolle, P. Experimental Animal Models in Periodontology: A Review. Open Dent J 2010, 4, 37–47, doi:10.2174/1874210601004010037.

Chen, X.; Wu, G.; Feng, Z.; Dong, Y.; Zhou, W.; Li, B.; Bai, S.; Zhao, Y. Advanced Biomaterials and Their Potential Applications in the Treatment of Periodontal Disease. Crit Rev Biotechnol 2016, 36, 760–775, doi:10.3109/07388551.2015.1035693.

Dobson, J.; Wilson, M. Sensitization of Oral Bacteria in Biofilms to Killing by Light from a Low-Power Laser. Arch Oral Biol 1992, 37, 883–887, doi:10.1016/0003-9969(92)90058-G.

Graves, D.T.; Fine, D.; Teng, Y.T.A.; van Dyke, T.E.; Hajishengallis, G. The Use of Rodent Models to Inves-tigate Host-Bacteria Interactions Related to Periodontal Diseases. J Clin Periodontol 2008, 35, 89–105, doi:10.1111/J.1600-051X.2007.01172.X.

Booij-Vrieling, H.E.; van der Reijden, W.A.; Houwers, D.J.; de Wit, W.E.A.J.; Bosch-Tijhof, C.J.; Penning, L.C.; van Winkelhoff, A.J.; Hazewinkel, H.A.W. Comparison of Periodontal Pathogens between Cats and Their Owners. Vet Microbiol 2010, 144, 147–152, doi:10.1016/J.VETMIC.2009.12.046.

Maeda, H.; Fujii, S.; Tomokiyo, A.; Wada, N.; Akamine, A. Periodontal Tissue Engineering: Defining the Triad. Int J Oral Maxillofac Implants 2013, 28, e461–e471, doi:10.11607/JOMI.TE26.

Oz, H.S.; Puleo, D.A. Animal Models for Periodontal Disease. J Biomed Biotechnol 2011, 2011, doi:10.1155/2011/754857.

García-Salinas, S.; Elizondo-Castillo, H.; Arruebo, M.; Mendoza, G.; Irusta, S. Evaluation of the Antimicrobial Activity and Cytotoxicity of Different Components of Natural Origin Present in Essential Oils. Molecules 2018, 23, doi:10.3390/molecules23061399.

Ripamonti, U.; Renton, L. Bone Morphogenetic Proteins and the Induction of Periodontal Tissue Regeneration. Periodontol 2000 2006, 41, 73–87, doi:10.1111/J.1600-0757.2006.00155.X.

Andersen, M.L.; Winter, L.M.F. Animal Models in Biological and Biomedical Research - Experimental and Ethical Concerns. An Acad Bras Cienc 2019, 91, doi:10.1590/0001-3765201720170238.

Partata Zuza, E.; Gouveia Garcia, V.; Helena Theodoro, L.; Ervolino, E.; Fernando Veloso Favero, L.; Longo, M.; Salimon Ribeiro, F.; Tadeu Martins, A.; Carlos Spolidorio, L.; Antônio Sampaio Zuanon, J.; et al. Influence of Obesity on Experimental Periodontitis in Rats: Histopathological, Histometric and Immunohistochemical Study., doi:10.1007/s00784-017-2207-y.

Ausenda, F.; Rasperini, G.; Acunzo, R.; Gorbunkova, A.; Pagni, G. New Perspectives in the Use of Biomaterials for Periodontal Regeneration. Materials (Basel) 2019, 12, doi:10.3390/MA12132197.

Charles, C.H.; Mostler, K.M.; Bartels, L.L.; Mankodi, S.M. Comparative Antiplaque and Antigingivitis Effec-tiveness of a Chlorhexidine and an Essential Oil Mouthrinse: 6-Month Clinical Trial. J Clin Periodontol 2004, 31, 878–884, doi:10.1111/J.1600-051X.2004.00578.X.

Hickman, D.L.; Swan, M. Use of a Body Condition Score Technique to Assess Health Status in a Rat Model of Polycystic Kidney Disease Available online: https://pubmed.ncbi.nlm.nih.gov/20353688/.

Dhingra, K.; Vandana, K.L. Indices for Measuring Periodontitis: A Literature Review. Int Dent J 2011, 61, 76–84, doi:10.1111/J.1875-595X.2011.00018.X.

Deng, N.; Xie, L.; Li, Y.; Lin, H.; Luo, R. Oxymatrine Alleviates Periodontitis in Rats by Inhibiting Inflam-matory Factor Secretion and Regulating MMPs/TIMP Protein Expression. Acta Cir Bras 2018, 33, 945–953, doi:10.1590/S0102-865020180110000001.

Turner, P. v.; Pang, D.S.J.; Lofgren, J.L.S. A Review of Pain Assessment Methods in Laboratory Rodents. Comp Med 2019, 69, 451–467, doi:10.30802/AALAS-CM-19-000042.

Katherine VARGAS-SANCHEZ, P.; Goetz MORO, M.; André dos SANTOS, F.; Lia ANBINDER, A.; Kreich, E.; Mendonça MORAES, R.; Padilha, L.; Kusiak, C.; Xavier SCOMPARIN, D.; Cesar Nobre FRANCO, G.; et al. (No Title). J Appl Oral Sci 2017, 25, 490–497, doi:10.1590/1678-7757-2016-0517.

Duarte, P.M.; Tezolin, K.R.; Figueiredo, L.C.; Feres, M.; Bastos, M.F. Microbial Profile of Ligature-Induced Periodontitis in Rats. Arch Oral Biol 2010, 55, 142–147, doi:10.1016/J.ARCHORALBIO.2009.10.006.

Aguirre, J.I.; Akhter, M.P.; Kimmel, D.B.; Pingel, J.; Xia, X.; Williams, A.; Jorgensen, M.; Edmonds, K.; Lee, J.Y.; Reinhard, M.K.; et al. Enhanced Alveolar Bone Loss in a Model of Non-Invasive Periodontitis in Rice Rats. Oral Dis 2012, 18, 459–468, doi:10.1111/J.1601-0825.2011.01893.X.

Zhang, W.; Ju, J.; Rigney, T.; Tribble, G. Porphyromonas Gingivalis Infection Increases Osteoclastic Bone Resorption and Osteoblastic Bone Formation in a Periodontitis Mouse Model. BMC Oral Health 2014, 14, 89, doi:10.1186/1472-6831-14-89.

Oktay, S.; Chukkapalli, S.S.; Rivera-Kweh, M.F.; Velsko, I.M.; Holliday, L.S.; Kesavalu, L. Periodontitis in Rats Induces Systemic Oxidative Stress That Is Controlled by Bone-Targeted Antiresorptives. J Periodontol 2015, 86, 137, doi:10.1902/JOP.2014.140302.

Downloads

Published

2022-11-15

How to Cite

Muresan, S., Dreanca, A., Nagy, A., Repciuc, C., Purdoiu, R. C., Pop, A. R., Pantea, S. and Oana, L. (2022) “The Histopathological and macroscopical considerations of induced experimental periodontitis in rats”, Cluj Veterinary Journal, 27(2), pp. 6–15. doi: 10.52331/cvj.v27i2.41.

Most read articles by the same author(s)