Decoding the Glycan Signature of Hyalinized Pancreatic Carcinoma in Dogs: Histochemical and Lectin-Based Evidence of Extracellular Matrix Heterogeneity
DOI:
https://doi.org/10.52331/v31i3xh43Keywords:
Hyalinizing pancreatic carcinoma, dog, lectin histochemistry, SNA, PHA-L, ECL, extracellular matrix , pancreasAbstract
Hyalinizing pancreatic carcinoma in dogs is a rare tumor characterized by abundant eosinophilic extracellular material of uncertain composition. The aim of this study was to characterize the nature of hyaline deposits using histochemical and lectin-based methods. Pancreatic tissues from three dogs (two Boxers and one American Staffordshire Terrier) were examined following necropsy. Sections were stained with hematoxylin and eosin, Masson’s Trichrome, and Congo Red, and analyzed using lectin histochemistry (SNA, PHA-L, ECL). All cases showed similar architecture, with neoplastic epithelial cells embedded in abundant hyaline stroma. Masson’s Trichrome demonstrated collagen-rich deposits in all cases. Congo Red staining revealed heterogeneity, with negative staining in two cases and weak to transitional positivity in one case, interpreted as equivocal and not sufficient to confirm amyloid deposition. Lectin histochemistry showed variable glycosylation patterns among tumors. These findings indicate that hyaline in canine pancreatic carcinoma is not a uniform entity, but a heterogeneous extracellular material composed predominantly of collagen-rich stroma and glycoprotein-associated matrix, with inter-case biochemical variability. Combined histochemical and lectin-based approaches provide additional insight into the molecular complexity of these lesions.
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Copyright (c) 2026 Ecaterina Semzenisi, Corina Toma, Haralambie Mara, Dragoș Hodor, Romelia Pop, Sorana Cătoi, Ibrahima Mamadou Sall, Alexia Teodora-Hoța, Alexandru Flaviu-Tăbaran
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